Isolation and identification of two bacteria from rotten Myrica rubra

JIANG Hui-fang; RAN Huo-miao; KONG Wang-jun; XIN Zhi-hong

doi:10.13386/j.issn1002-0306.2016.10.044

JIANG Hui-fang, RAN Huo-miao, KONG Wang-jun, XIN Zhi-hong. Isolation and identification of two bacteria from rotten Myrica rubra[J]. Science and Technology of Food Industry, 2016, (10): 260-264. DOI: 10.13386/j.issn1002-0306.2016.10.044

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Isolation and identification of two bacteria from rotten Myrica rubra

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In this study,two strains of bacteria coded as YMS-7 and YMN-5 were isolated from rotten Myrica rubra using plate streaking and slant culture methods.After extraction of DNA,16 S r DNA from both strains were amplified for sequencing and homology analysis using Ez Bio Cloud database.Two phylogenetic trees were established based on these sequences of 16 S r DNA,combined with physiological and biochemical characteristics. Consequently,YMS-7 and YMN-5 were identified as Paenibacillus agaridevorans and Bacillus aerophilus,respectively.

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Isolation and identification of two bacteria from rotten Myrica rubra

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