Based on high- throughput sequencing analysis of bacterial community structure in Yibin sprouts

Objective: The aim of this study was to analyze the yibin sprouts bacteria microbial diversity in the process of fermentation.Methods: Illumina high- throughput sequencing techno- logy were used to sequence 16 S V1- V3 hypervariable reqion of all 6 yibin sprouts samples.QIIME and Mothur was used to analyze and calculate the numbers of sequences and operational taxonomic unitl( OTUs) for each sample,and followed by cluster analysis.Results: Yibin sprouts 6 stages in the process of fermentation samples received 204997 valid sequence,the optimized effectively sequence number was 41703,a total of 9202 OTUs.The main groups for bacteria of Yibin sprouts was Bacillus under the Firmicutes,Flavobacteriaceae and Sphingobacterium under the Bacteroidetes,Sphingomonas,Acidovorax,Chromohalobacter and Pseudomonas under the Proteobacteria,and Arthrobacter under the Actinobacteria.Conclusion: Yibin sprouts bacterial flora in the fermentation process of dynamic change,with the fermentation proceeds,some bacteria gradually decreased or disappeared,dominant bacteria stabilized.