Application of phytase on the extraction of rice bran gluten

The residue was used as experiment material,after albumin,globulin,prolamin were removed from defatted rice bran. Single factor experiments were conducted to explor effects of four reaction parameters,including temperature,p H,time and phytase addition amount on degradation of phytic acid in rice bran.Based on this,the response surface methodology was designed according to the principle of Box- Benhnken central combination design. The results showed that the optimal conditions were obtained as follows: phytase addition dosage 2.5 U / g,p H5.6,reaction time was 3 h,reaction temperature was 48 ℃. The practical phytic acid removal rate was 85.47%.Under the optimal conditions,the extraction yield and purity of rice bran gluten were increased by24.53% and 10.16% respectively.At the same time,phytic acid content in gluten was reduced by 93.2% and the solubility of gluten improved to a certain extent.