Optimization decoloration process of Agaricus Blazei Murill polysaccharide by response surface methodology

Response surface methodology was utilized to optimize the decoloration process of Agaricus Blazei Murill( ABM) polysaccharide.Based on single factor test,the mass concentration of sample,sample volume and elution flow rate of ABM polysaccharide decoloration process were selected as influential factors.A three- variable,threelevel Box- Behnken experiment design was combined with response surface analysis.And the normalized values( OD) of ABM polysaccharide decoloration process of decolorization rate and polysaccharide retention rate was selected as the response value.The results indicated that the optimal decoloration process conditions were determined as 40 mg / m L,3 m L and 2 BV / h for sample mass concentration,sample volume and elution flow rate,respectively.Under these conditions,the decolorization rate was 88.5% and polysaccharide retention rate was69.5%,the predicted values of the model was 0.47 and the normalized values( OD) of actual value was 0.48,which showed a small error between both.Thus,the newly optimized decoloration process conditions are feasible.