Optimizing of composite-enzymolysis assisted extraction process of green tea polyphenols

Using cellulase and pectase as a mean of stimulus, composite- enzymolysis assisted extraction process of polyphenols from green tea was optimized in this investigation. The effects of factors as enzyme content,solid-to-liquid ratio,enzymolysis temperature,extraction time on the extraction rates of tea polyphenols were explored through single-factor and orthogonal test. The results showed that the optimal conditions were addition of 0.3% of cellulase and pectase respectively,solid-to-liquid ratio 1∶40 g/m L,enzymolysis temperature50 ℃,enzymolysis time 45 min.Under such optimum conditions,extraction rate of green tea polyphenols reached up to 21.36% which was much higher than 17.41% obtained from traditional water extraction.