Purification of total flavonoids from juniperus sabina l.by macroporous resin

By comparing the static adsorption effect of D101,AB- 8,HPD400,HPD500,HPD417 and HPD826 macroporous resin,resin was screened out for separating total flavonoids from Juniperus sabina L.And purification process of total flavonoids from Juniperus sabina L. was optimized by orthogonal exceperiments based on single factor exceperiments. the results showed that D101 macroporous resin for total flavonoids had good separation effect.The optimal parameters for purification were as follows: the sample concentration of 1.2256 mg / m L,the sample flow rate of 1.0 m L / min,water consumption on removing impurity of 5 BV,ethanol concentration of 50%,eluting agent volume of 4 BV,desorption rate of 1.0 m L / min.Under these conditions total flavonoids had a recovery rate of 88.36% and a purity of 69.96%.