Breeding of oxygen- resistant Bifidobacterium longum and its high- density fermentation conditions

LV Xiu- ming; LIANG Jin- zhong

doi:10.13386/j.issn1002-0306.2016.07.023

LV Xiu- ming, LIANG Jin- zhong. Breeding of oxygen- resistant Bifidobacterium longum and its high- density fermentation conditions[J]. Science and Technology of Food Industry, 2016, (07): 159-163. DOI: 10.13386/j.issn1002-0306.2016.07.023

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Breeding of oxygen- resistant Bifidobacterium longum and its high- density fermentation conditions

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Through the method of ultraviolet mutagenesis to Bifidobacterium longum,a strain was screened with resistance ability to oxygen,the strain was named L80. Using L80 as the test strain,the optimum culture medium was determined by single factor,response surface and flow and feeding method. High density culture medium optimization formula was( mass fraction) soy peptone 4.03%,yeast extract powder 2.02%,glucose 0.95% and stachyose 1.59%.The optimal fed- batch cultivation was: the sugar concentration was kept in 6.0 ~8.0 g / L,p H6.5,and the final viable bacteria number was reached( 1.21 ± 0.03) × 10¹⁰ cfu / m L.

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Science and Technology of Food Industry

Breeding of oxygen- resistant Bifidobacterium longum and its high- density fermentation conditions

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