Effect of bifunctional mesoporous material on Porcine trypsin catalytic properties

In this paper,binding force between mesoporous materials and trypsin was improved by introducing different modifiers functional groups on the surface of SBA-15(- SH,- Cl,- NH₂,-C₁,-C₃,-C₈ and- C₁₆) in order to increase the efficiency of enzyme loading,activity and stability.Activity of PT- NH₂/ C₈- SBA could be found up to255 U / mg trypsin,the activity of PT- SBA was just 195 U / mg trypsin. After the fifth recycle and storage at room temprature of 5 d,PT- NH₂/ C₈- SBA was still able to maintain 85% and 81% of initial activity,while the PT- SBA retained only 51% and 67% of the initial activity.Kinetic studies showed that immobilized enzyme PT- NH₂/ C₈- SBA has better affinity with substrate.