Study on extraction,purification and antitumor activity of polysaccharide from bee pollen of Dendranthema indicum

The purification procedures,physiochemical features and antitumor activity of polysaccharide fractions from bee pollen of Dendranthema indicum were studied in this paper.Polysaccharide fractions( WDPP) was dealed with water-extraction,ethnol-precipetation and ion- exchange chromatography,then one netral( WDPP- N) and two acidic fractions( WDPP-1,WDPP-2) were obtained.The monosaccharide composition was tested through HPLC,the antitumor activity of all fractions were investigated by MTT method.WDPP-N was composed of galactose,arabinose,and glucose with a ratio of 30.3 ∶ 31.6 ∶ 32.7; WDPP- 1 and WDPP- 2 were mainly composed by galacturonic acid,galactose and arabinose,rhamose and glucose as small proportion.Total polysaccharide had obvious inhibition effect on HT-29 and HCT116 tumor cells in high concentration compared with neutral and acidic fractions.