Determination of the polysaccharide content in origin peach gum by ion chromatography with pulsed ampere detector

The ion chromatographic method with pulsed amperometric detectorhad was developed for the determination of peach gum polysaccharide content( rhamnose,arabinose,galactose,glucose,mannose). The analytes were separated on a METROSEP CARB 1( 150 mm × 4.0 mm) anion exchange column with 10 mmol / L Na OH as mobile phase at the flow rate of 1.0 m L / min,and the total analysis time of 22 min. The linear range of rhamnose and mannose was 5.0 to 100.0 μg / m L,the linear range of arabinose,galactose,glucose line arrange was2.0 to 60.0 μg / m L. The detection limits of rhamnose,arabinose,galactose,glucose and mannose( 20 μL injection,S / N = 3) were 0.34,0.18,0.17,0.08,0.39 μg / m L,respectively. The recovery rates ranged from 92.0% to 98.3%,and RSDs of 5 polysaccharide contents ranged from 2.25% to 4.23% after 6 consecutive injections. Therefore,the proposed determination method was derivative- free,simple,fast,effective and sensitive,which facilitates the rapid detection and effective quality control of polysaccharide contents in peach gum.