Optimization technology of composite enzyme hydrolysis of Nanguo pear juice by response surface methodology

Nanguo pear was used as raw material,the optimal technical process of complex enzyme extracting juice was researched.The effect of composite enzyme dosage,enzyme proportion,enzymolysis time and enzymolysis temperature on Nanguo pear juice yield and soluble solids content were studied respectively by single factor experiment.Based on the above results,in order to improve Nanguo pear juice yield and soluble solids content,complex enzyme enzymolysis processing parameters were optimized by Box- Behnken design method.The results showed that the optimal parameters were as follows: 0.15%,119 min,3 ∶ 1,42 ℃ for composite enzyme dosage,enzymolysis time,enzyme proportion,enzymolysis temperature,respectively. Under the condition,the juice yield was 78.15% with the relative error of 0.93% which was consistent with the prediction value 77.42% from response surface fitting equation with the relative error of 0.93%.Compared to the group without enzyme treatment,the juice yield and the soluble solid content were increased by 12.92% and 1.72%,respectively.From the test result,we got that related physical and chemical indicators such as juice yield and the soluble solids content could significantly increase after compound enzyme enzymolysis.