Optimizing techniques of Perilla seed oil by aqueous enzymatic extraction assisted with heat treatment

Perilla seed was used as raw material,aqueous enzymatic extraction assisted with heat treatment as the technique. On the basis of one factor test,enzymolysis temperature,time,enzyme loading and p H were chosen as independent variable,the yield of free oil as response value. Then the response surface methodology was applied to test,the effects of every independent variable as well as their interaction on the yield of free oil were researched. The results showed the orders which influenced the free oil yield were as follows:enzyme loading >enzymolysis temperature >enzymolysis time >p H. And the optimized enzymolysis conditions were enzymolysis temperature 46 ℃,enzymolysis time 3.0 h,enzyme loading 3.49%(cellulase∶neutral protease=1∶2),p H6.0. The proof test gained the free oil yield was 59.02%,and compared with predicted value,the relative error was 1.49%. It suggested that the technical parameters optimized by test were reliable.