Comparison of microbial genome DNA extraction methods used in the fermentation process of northwestern Shanxi acid gruel

In order to obtain a simple and efficient extraction method of microbial genome DNA from northwestern Shanxi acid gruel,three extraction methods(lysozyme-SDS-proteinase K method,improved CTAB method and improved CTAB-lysozyme method) were compared according to the concentrations of extracted microbial genome DNA. The results showed that the effect of the lysozyme-SDS-proteinase K method was the best,flowed by improved CTAB-lysozyme method and improved CTAB method. By lysozyme-SDS-proteinase K method,higher quality of microbial DNA could be obtained and the DNA concentration was 1657.53 ng/μL.When using the improved CTAB-lysozyme method and improved CTAB method,the DNA concentrations were respectively 1308.08 ng/μL and 1098.36 ng/μL. The total DNA of 16 S r DNA gene PCR amplification was tested.The results showed that the purpose stripe was clear in recycling products,which indicated that the microbial genome DNA obtained in this experiment had high content and complete structure. All the results showed that the lysozyme-SDS-proteinase K method was the best to extract the microbial genome DNA of northwestern Shanxi acid gruel.