Investigation of antibiotic resistance and resistant genes of epibiotic lactic acid bacteria on the surface of Sichuan pickled cowpea

The epibiotic lactic acid bacteria(LABs) on the fresh cowpea,which resist to chloramphenicol(CHL)tetracycline(TET) and ampicillin(AMP),were investigated by the physiological and biochemical characteristics,16 S r RNA,antibiotic resistance and resistant genes. 182 isolates in current study were assigned to Weissella confuse(50.0%),Weissella cibaria(15.4%),Enterococcus sulfurous(7.1%) and Lactococcus lactis subsp. lactis( 27. 5 %), respectively. And 32 isolates( 17. 6 %) were found to be against CHL and TET with one or two resistance,including 6 W. confuse(3.3%) and 4 L. lactis subsp. lactis(2.1%) with resistance to CHL,and 4W. confuse(2.1%) and 5 L. lactis subsp. lactis(2.7%) with resistance to TET. 6 W. confuse(3.2%),2 W. cibaria(1.1%),1 E. sulfurous(0.5%) and 4 L. lactis subsp. lactis(2.1%) with resistances to CHL and TET. In the effux pump genes efr A,efr B,nor B,nor C,nor E and sug E,efr B had the highest detection rate with 31.3%,but efr B and sug E with only 3.1%. Among the TET resistant genes tet(A),tet(B),tet(C),tet(D),tet(H) and tet(L),tet(C)had the highest detection rate,90.9%,and tet(B) was found with 4.5% lowest detection rate.