Ultrasonic- assisted anionic and cationic surfactant systems extraction of total flavonoids from Ampelopsis grossedentata

The ultrasonic- assisted anionic and cationic surfactant systems extraction of total flavonoids was optimized by single- factor experiment and response surface methodology.The effects of surfactant concentration,the ratio of anionic surfactant to cationic surfactant,ultrasonic treatment time,solid / liquid ratio( g / m L) and temperature on the yield of total flavonoids were explored through single factor.Based on the optimum conditions of single factor experiment as SDS ∶ CTAB = 40 ∶ 1、1 ∶ 20 and 60 ℃ for the ratio of anionic surfactant to cationic surfactant,solid / liquid ratio( g / m L) and temperature,The optimum extraction conditions were determined as 1.1%,40% and 57 min for surfactant concentration,ethanol concentration and extraction time,respectively.Under these conditions,the yield of total flavonoids from Ampelopsis grossedentata was 23.27%,which was close to the predicted value of 23.45%.Therefore,the optimized extraction procedure was reliable.