Study on the cloning and prokaryotic soluble expression of Lipase gene Lip2 of high- yield isoamyl acetate strain

TAN Cai- deng; ZHU Mei- juan; LIAO Yan- zhi; ZHU Xiao- li

doi:10.13386/j.issn1002-0306.2016.01.023

TAN Cai- deng, ZHU Mei- juan, LIAO Yan- zhi, ZHU Xiao- li. Study on the cloning and prokaryotic soluble expression of Lipase gene Lip2 of high- yield isoamyl acetate strain[J]. Science and Technology of Food Industry, 2016, (01): 153-155. DOI: 10.13386/j.issn1002-0306.2016.01.023

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Study on the cloning and prokaryotic soluble expression of Lipase gene Lip2 of high- yield isoamyl acetate strain

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The gene encoding Lipase2( Lip2) from high- yield isoamyl acetate strain Loq- c was amplified by polymerase chain reaction( PCR),its sequence was most similar to Candida cylindracea Lip2 gene( X64704.1),the similarity was 89%.Subsequently,the corresponding expression vector p QE- lip2 was constructed and cloned into E.coli BL21( DE3),resulting in recombinant strain BL21( p QE- lip2).After explorations,SDS- PAGE analysis showed that the best conditions for soluble expression of Lip2 was: 30 ℃,IPTG 1.5 mmol / L,150 r / min,induced for 5 hours.

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Science and Technology of Food Industry

Study on the cloning and prokaryotic soluble expression of Lipase gene Lip2 of high- yield isoamyl acetate strain

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