Study on the ultrahigh pressure inactivation kinetics of pork superoxide dismutase

The superoxide dismutase( SOD) activity of pork under different pressure( 400 ~750 MPa) combined with different tempreture( 20 ~ 60 ℃) were determined,and the ultrahigh- pressure inactivation kinetics of SOD was researched in the further study. The results showed that at a constant temperature( 20 ℃ ≤ T ≤ 60 ℃),the deactivation constants of SOD in the pork was increased with the increasing of pressure. The reaction activation volume Vawas negative in the various temperature conditions,which showed that the inactivation constants of SOD was increased with the increasing of pressure.The absolute value of SOD reaction activation volume was minimum at 40 ℃ and 60 ℃,which showed that the pressure had the minimal effect on ultrahigh- pressure inactivation of SOD,and SOD had a high pressure stability in these temperature conditions. Throughout the test pressure range( 400 Mpa≤P≤750 Mpa),the activation energy Eavalues were decreased generally,which indicated that the influence of temperature on SOD inactivation constants were weakening with the increasing of pressure.