Purification and preparation of geniposidic acid by macroporous resin

The factors and process conditions on purification and preparation of geniposidic acid from alkaline hydrolyzate of exhausted solution of gardenia yellow by macroporous resin were studied.The optimum technology of the preparation of GPA was found according to the decolorization and adsorption- desorption performance of GPA by different resins.The results showed that DA201 weak polar resins had the best decolorization performance and poor absorption of the GPA; DA201 C non- polar resin had higer adsorption rate and desorption rate,reaching88.7% and 98%,respectively.When the amount of resin was 1 g /25 m L 10 mg / m L of GPA crude liquid,p H 4.0,25 ℃,120 r / min,decoloration time 6 h,the decolorization rate reached 85.9%,GPA retention rate reached 92.3%.The optimum conditions for DA201 C resin adsorption of GPA were as follows: destaining solution concentration10 mg / m L,p H 3.0,adsorption velocity 1 BV / h,sample volume 7.2 BV.After eluting with 25% ethanol and drying the eluent,a purity of GPA of 95.1% and a recovery rate of 86.2% were obtained. It showed that DA201 resin was suitable for decolorization and DA201 C resin was suitable for adsorption- desorption of GPA on purification and preparation.