Optimization of conjugate conversion rate of linoleic acid in okra seed oil by response surface method

In order to improve the conjugate conversion rate of linoleic acid in okra seed oil,the Box-Benhnken experimental design was performed on the basis of one-factor experiments,and the response surface analysis and corresponding regression model were established with conjugated linoleic acid(CLA) yield as response value. The optimum conditions of conjugate conversion were obtained as follows:reaction time of 4 h,reaction temperature of 162 ℃,oil against KOH at ratio of 1.9(m L/g),propylene glycol against oil at ratio of 2.0(m L/m L),and the conversion rate of CLA was 74.20%. Conversion rate was greatly affected by reaction temperature and the interaction of reaction time and temperature was significant. The measured date( 74.20%) was almost accorded with the predicted data(76.99%). It demonstrates that the conversion condition parameter optimized by response surface method was credible and feasible.