Optimization of liquid culture conditions and fruiting bodies culture of Xylaria schweinitzii

Objective:Based on experiments,this research attempted to find out the optimal nutritional conditions,optimal growth environment for Xylaria schweinitzii mycelium. It also explored the best formula of solid substrate for its cultured fruiting body. Methods : Using mycelium biomass as the indicator, single- factor experiments were conducted in an effort to study the effects of different carbon sources,nitrogen sources,mineral salts,temperature,p H and liquid volume used in the solid substrate on the growth of cultured fruiting body Xylaria schweinitzii. Biological efficiency was used to measure the effects of different formula of solid substrate on cultured fruiting bodies of Xylaria schweinitzii. Results:Results showed that lactose was the optimum carbon source,and peptone was the optimum nitrogen source,and mineral salt which was Mn SO4,KH2PO4,Mg SO4,K2SO4 had positive effects in promoting the growth of mycelium. It was also found out that the optimal temperature was 30 ℃,the optimum p H for fermentation was 7,the optimum liquid volume was 50 m L and optimal solid substrate consists of 68% sawdust and 30% bran. When the optimal formula was used,the biological efficiency reached 8.14%(w/w,dry). Conclusion:This research tried out a liquid culture method for culturing Xylaria schweinitzii. And for the first time,this method was successfully used to culture fruiting bodies of Xylaria schweinitzii.