TANG Cheng-cheng, QIU Zhi-dong, YANG Jing, DONG Jin-xiang. Study on quality standard and fingerprint of American ginseng granule[J]. Science and Technology of Food Industry, 2015, (24): 71-75. DOI: 10.13386/j.issn1002-0306.2015.24.006
Citation: TANG Cheng-cheng, QIU Zhi-dong, YANG Jing, DONG Jin-xiang. Study on quality standard and fingerprint of American ginseng granule[J]. Science and Technology of Food Industry, 2015, (24): 71-75. DOI: 10.13386/j.issn1002-0306.2015.24.006

Study on quality standard and fingerprint of American ginseng granule

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  • Received Date: May 01, 2015
  • Objective:To establish the quality control method of American ginseng granule. Methods:10 batches of American ginseng from different regions were identified by TLC and made into American ginseng granules which were established quality standard by HPLC fingerprint. The HPLC condition was that:ZORBAX SB-C18(250 mm×4.6 mm,5 μm) column,gradient elution of mobile phase of acetonitrile-0.1% phosphoric acid solution,203 nm detection wavelength,25 ℃ column temperature. Results:The TLC method showed well specificity,clear spots with no negative interference. The fine linear ranges of Ginsenoside Rg1,Re and Rb1 were 0.0794~0.4764 μg/m L(r =1),0.318 ~1.908 μg/m L(r =0.9998),0.8216 ~4.9296 μg/m L(r =0.9999) respectively. The fingerprint of American ginseng was established with 13 common peaks and a similarity between 0.944 to 0.988.Conclusion:The method was accurate with good reproducibility. HPLC fingerprint provided a comprehensive reflection on identification and quality evaluation of the granule.
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