The specific detection research of nuts allergen with indirect-competitive ELISA

The methods of the indirect competitive ELISA for determining allergen in nuts were developed by using the self-prepared positive mice serum as a resistance in Cashew protein sensitization. The immunoglubing Ig E- HRP for 2 resistance was adopted. Cashew protein was identified through checkerboard microlilution method,the optimal solution was 15.92 μg/m L,the equal dilution multiples of mice Ig E and antibody-HRP were400 and 1000,cashew protein concentration was 1.99 μg/m L. Using the method of cashew nuts and walnuts,hazelnuts,pistachios,chestnut,almond nuts,six test cross reaction in nuts analysis had not been found. The method for rapid detection to allergen about nuts was established firstly by using and sensitivity and reliability,accurately specificity.