Development of an immunochromatographic test strip for the rapid detection of fumonisin B1

Objective : An immunochromatographic test strip( ICG) had been developed for rapid detection of fumonisin B1 residues in maize samples. Methods:For this purpose,FB₁ coupled to bovine serum albumin(BSA) via the modified EDCA method was prepared as capture antigen. The colloidal gold was prepared by the reduction of tetrachloroauric(III) acid trihydrate with citric acid trisodium salt. Results :Using an antibody purified by the ammonium sulfate precipitation,and a titer of the antibody was about 1.28 ×105by ELISA. The ICG was composed of NC membrane,sample pad,probe pad(colloidal gold-monoclonal antibody probes for FB₁ and absorbent pad. Moreover,the sensitivity,specificity and stability of the ICG were also detected. A total of 0.74 μL/cm of FB₁-BSA(200 μg/m L) and the goat anti-mouse Ig G antibody(1.0 mg/m L) were sprayed onto the NC membrane as the test(T line) and control lines(C line),respectively. The sensitivity of the test strip was20 ng/m L. The test could be accomplished within 5 min. The stability of the ICG was about 12 months at 4℃.Conclusion:Analysis of FB₁ in maize samples revealed that data obtained from the ICG were in good agreement with those obtained from HPLC and ELISA. The results demonstrated that the ICG could be used as qualitative tool for rapid screening of FB₁on-site.