Establishment of a PCR method with an internal amplication control for rapid detection of Salmonlla in foods

A PCR assay was developed for the rapid detection of Salmonlla using 16S rRNA as internal amplification control. Primers were designed to detect the gene of inv A in Salmonlla and the assay was optimized and assessment. Detection of various bacterias by PCR indicated that this PCR assay was specific for Salmonlla.The sensitivity of detection for Salmonella purified genomic DNA was 61.1 fg/μL and 2 ×102cfu/m L for pure cultures. The detection for artificially contaminated egg white showed that Salmonlla could be detected after eight hours enrichment culture when the Sallmonla inoculation was 2 cfu/25 mL. This detection method had a good specificity and sensitivity,and could eliminate false-negative results in the PCR detection method for Salmonella,and suitable for rapid detection of Salmonella in food.