Science and Technology of Food Industry

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HAO Lu-jiang, FAN Qiu-ping, ZHANG Xiao-fei, LU Xiao-ping, XU Yan-rui. Optimization of fermentation conditions for exopolysaccharide EPS- PⅡby marine bacterium Pseudoalteromonas issachenkonii[J]. Science and Technology of Food Industry, 2015, (23): 256-260. DOI: 10.13386/j.issn1002-0306.2015.23.044
Citation: HAO Lu-jiang, FAN Qiu-ping, ZHANG Xiao-fei, LU Xiao-ping, XU Yan-rui. Optimization of fermentation conditions for exopolysaccharide EPS- PⅡby marine bacterium Pseudoalteromonas issachenkonii[J]. Science and Technology of Food Industry, 2015, (23): 256-260. DOI: 10.13386/j.issn1002-0306.2015.23.044
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Optimization of fermentation conditions for exopolysaccharide EPS- PⅡby marine bacterium Pseudoalteromonas issachenkonii

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  • Objective: To optimize the fermentation conditions of exopolysaccharide EPS- P Ⅱ produced by Pseudoalteromonas issachenkonii.Method: The medium composition was determined by single factor experiment,Plackett- Burman combined with Box- Behnken was used to determine the optimum fermentation conditions.Results: The experiment showed that the fermentation medium was composed of glucose,yeast extract and baysalt,the fermentation conditions were 8.8% inoculum,170 r / min shaking speed and 33.8 h for fermentation.Conclusion: Under the optimum conditions,the yield of EPS- PⅡwas 2.62 g / L,which was increased by 116.5%.
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