Cloning and sequence analysis of the FZF1 gene concerning sulfur tolerance from Saccharomyces bayanus

The FZF1 gene of Saccharomyces cerevisiae was found to be a positive regulator of SSU1 transcription,but there was no report about the FZF1 gene of Saccharomyces bayanus.In this study,the genes were cloned and the content of bioinformatics was analyzed by online tools for setting the foundation of the further research.Here the FZF1 gene of S.bayanus was cloned and the physical and chemical properties of FZF1 protein were analyzed by online analytical tools,such as Prot Param,Prot Scale,TMHMM,Predict Protein and Swiss- Model.At the same time,the protein secondary structure and tertiary structures were predicted. The results indicated that the FZF1 gene contained an opening reading frame( ORF) of 900 bp encoding a 299 predicted amino acids. The protein was a hydrophilic protein exercising their control functions in the nucleus. It contained 18 serine kinase potential phosphorylation sites,a coil domain and 4 zinc finger domains.The protein encoded by FZF1 gene of S.cerevisiae was very similar with the predicted protein.So the FZF1 gene of S.bayanus was inferred to be associated with the sulfur resistance.And the protein encoded by the FZF1 gene of S.bayanus only contains 4 zinc finger domains.It was one of the most important reasons that the sulfur tolerance of most S.bayanus was weaker than most S.cerevisiae.