Study on the distribution and the extraction process of rich proteins of Xinchun No.36 Triticale in Xinjiang province

The purpose of this work was to study the distribution of Se in Xinchun No.36 triticale in Xingjiang province. Through single factor experiment and response surface method, a systematic inspection of selenoproteins,selenium polysaccharide,selenium nucleic acid and selenoproteins of solubility in different solvents was made. The results showed that content of selenoproteins,selenium ploysaccharide and nucleic acid selenium was(240.52±0.3),(32.45±0.21) and(4.65±0.05) mg/g respectively. The content of selenoproteins of alkali-soluble,water-soluble,salt-soluble and alcohol-soluble was(146.21±0.3),(41.52±0.6),(19.40±0.06)and(13.65±0.12) mg/g respectively. Furthermore,in order to get more alkali-soluble selenoproteins,the optimal extraction technology was obtained by the single factor test and the response surface design experiments. The optimal extraction conditions were Na OH 0.17 mol/L,acidification at p H4.7,saturation of(NH4)2SO466.0%,the ratio of solid to liquid 1 ∶10, the predicted rate of protein extraction was 76.5593%, but the actual value of experiment reached 76.7%.