Optimization of microwave extraction of estradiol in milk by response surface methodology

The extraction process of estradiol from milk was investigated in this paper. Based on single factor experiments,response surface methodology was used to explore the effects of liquid-material ratio,microwave power and microwave time on extraction yield of estradiol. The results showed that the optimal conditions for the extraction of estradiol were the liquid-material ratio of 4.1∶1,microwave power of 475 W,microwave time of61 s. Under the optimal extraction conditions,the highest extraction rate of estradiol was up to 92.77%. Compared with the ultrasonic extraction method,microwave extraction method had short extraction time,high extraction yield,low energy consumption,environmental protection and other advantages.