Bienzymatic hydrolysis process of sika deer antler base

Antler base as a traditional Chinese medicine, is believed to nourish liver, tonify, kidney, invigorate spleen and stomach,strengthen bones and muscles,promote blood flow and treat mastitis early. In this paper,antlers base powder as raw material,the degree of hydrolysis was as a measure of the optimal indicators of the extraction process. And the best combination of dual enzymatic hydrolysis of peptides was selected and obtained. Based on one-factor-at-a-time method,the response surface(RSM) analysis method based on Box-Behnken design was applied. The result of determining the optimal level of enzyme hydrolysis process is:temperature of 49 ℃,p H of 8.0,270 min with enzyme dosage of 11000 U/g,enzyme activity ratio of(papain∶trypsin) 2∶1 and a substrate concentration of 6 g/100 m L. Under this condition,the hydrolysis degree could be reached 15.91%,respectively.