Isolation,identification and enzyme characteristics of the chitosanase producing marine fungal MF-08

LI Jia-yin; WANG Hui-min; ZU Guo-ren

doi:10.13386/j.issn1002-0306.2015.22.032

LI Jia-yin, WANG Hui-min, ZU Guo-ren. Isolation,identification and enzyme characteristics of the chitosanase producing marine fungal MF-08[J]. Science and Technology of Food Industry, 2015, (22): 193-197. DOI: 10.13386/j.issn1002-0306.2015.22.032

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Isolation,identification and enzyme characteristics of the chitosanase producing marine fungal MF-08

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Isolated and purified a high enzyme activity strain from the chitosanase producing Marine fungi which preservation in laboratory,it was named MF-08. Based on the 26 S r DNA sequence analysis and morphological properties,Marine fungi MF-08 was assessed to be Aspergillus flavus. The optimum temperature of chitosanase was 40 ℃,the optimum p H was 5.2,the chitosanase was stable below 40 ℃ and p H4.0~6.0,respectively. The enzyme activity was inhibited by Cu2+,Fe3+and Hg2+.

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Isolation,identification and enzyme characteristics of the chitosanase producing marine fungal MF-08

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