Breeding of Monascus with high-yield yellow pigment by 24 well-plate assay combining with mutation

YANG Qiang; YUE Jian-ming; WANG Cheng-tao; WEN Yan-jun

doi:10.13386/j.issn1002-0306.2015.22.024

YANG Qiang, YUE Jian-ming, WANG Cheng-tao, WEN Yan-jun. Breeding of Monascus with high-yield yellow pigment by 24 well-plate assay combining with mutation[J]. Science and Technology of Food Industry, 2015, (22): 156-159. DOI: 10.13386/j.issn1002-0306.2015.22.024

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Breeding of Monascus with high-yield yellow pigment by 24 well-plate assay combining with mutation

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Monascus F9-2 was used as the original strain;then the high-yield yellow pigment of Monascus Y16 strain was obtained by UV(130 s) and Li Cl(0.1%) complex mutation combining with the 24 well-plate assay.The results showed that the yellow pigment value of Monascus Y16 could reach 488 U/m L and the value was37.1% higher than the starting strain. The stable genotype was verified through ten times of transferring of culture and was hence a fine strain for producing high-yield yellow pigment.

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Science and Technology of Food Industry

Breeding of Monascus with high-yield yellow pigment by 24 well-plate assay combining with mutation

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