Antioxidant property of wild Lilium species from Qin-ba mountain

Three different habitat of L. leucanthum,L. concolor,L. pumilum,L. lancifolium,L. regale and L. davidii var. unicolor were evaluated for total polyphenol content by Folin-Ciocalteau method,the total flavonoid content content was measured using Na NO2-Al Cl3 colorimetric method and the determination of total flavanol content was performed colorimetrically by the vanillin method. The scavenging ability of DPPH· and ·OH,the cupricion reducing capacity, lipid peroxidation inhibition and metal chelating capacity were used to evaluate in vitro antioxidant activity of lily bulbs. The results showed that the contents of total polyphenolics and antioxidant activities of the wild Lilium species were significantly higher than those of L. davidii var. unicolor(p<0.05).Furthermore,there was a significant dose-effect relationship between the contents of lily bulb extracts and their antioxidant activities. Furthermore,the phenolic compounds of bulbs from tested Lilium species exhibited a significant positive correlation with antioxidant capacity(p<0.05 or p<0.01),and the positive correlation among the different methods was found(p <0.05). In addition,there was a significant differences among the three different habitat of L. leucanthum for their polyphenolis compounds and antioxidant activities(p<0.05). The Xixiang ecotype of the selected L. leucanthum had the highest contents of total polyphenolics and total flavonoid,and showed the strongest cupric-reducing antioxidant capacity. The Liuba ecotype of L. leucanthum had the highest total was flavanol content with the most outstanding DPPH free radical scavenging activity, metal chelating capacity and lipid peroxidation inhibition. The strongest hydroxyl radical scavenging activity was found in Zhouqu ecotype of L. leucanthum bulb extracts. These results strongly suggested that it was necessary to select propriate provenances for the utilization of wild Lilium species.