Study on the inhibitory effect and inhibition mechanism on α-glycosidase of ethanol extract from Zanthoxylum armatum DC.

Objective: To investigate different ethanol extract of Zanthoxylum armatum DC. on inhibitory activity to a- glucosidase. Method: The inhibitory activity of these ginsenosides for the a- glucosidase and a- amylase were determined by in vitro experiments. Inhibitory kinetic parameters were determined by Lineweaver- Burk plot.Results: The median inhibitory concentration ( IC50) of ethanol extract was ( 0.660 ± 0.145) mg ·m L- 1onα- glucosidase, ( 1.185 ± 0.132) mg·m L- 1on α- amylase and ( 1.944 ± 0.078) mg·m L- 1on α- glucosidase from small intestine in mice. Kinetic analysis showed that the inhibitory mechanism of Zanthoxylum armatum DC. onα- glucosidase was a typical noncompetitive inhibition. Conclusion: Ethanol extract of Zanthoxylum armatum DC.have remarkable inhibitory effect to α- glucosidase activity.