Box- Behnken design response surface methodology to optimize process of squid rehydration

Three extraction parameters including the addition amount of alkali, treatment time of alkali and treatment temperature of alkali were optimized using the Box- Behnken central composite design optimization for achieving maximum sensory scores, rehydration ratio and minimum the amount of soluble protein loss. The rehydrated squid optimum conditions were the amount of 0.33% alkali, alkaline reaction time of 8h, alkali effect temperature 25 ℃.Under these conditions, sensory score of the rehydration squid was 83.41, rehydration ratio was 2.96, the amount of soluble protein loss was 9.835 mg / g.Compared to the theoretical value that sensory score of the rehydration squid was 82.10, rehydration ratio was 2.89, the amount of soluble protein loss was 10.094 mg / g, the relative error were1.57%, 2.36% and 2.63%, respectively. Regression equation optimized by response surface derived some practical significance.