Research of the antioxidant activity of Chrysanthemum Morifolium's extracts with different solvents

In present work, the components of Chrysanthemum were extracted by water, 80% ethanol, ethyl acetate and petroleum ether with different polarity respectively. The total reduction ability, DPPH·clearance rate, O-2·clearance rate and ·OH clearance rate were studied as indexes to evaluate the antioxidant ability of four kinds of extracts of Chrysanthemum.The results showed that the order sequenced by the total reduction ability from strong to weak was: 80% ethanol extract > ethyl acetate extract > water extract > petroleum ether extract.The components extracted by 80% ethanol had the strongest capacity of scavenging free radical and the IC50 of freeing DPPH·, O-2·and ·OH were 0.33, 0.44, 0.34 mg /m L respectively.The components extracted by petroleum had the weakest ability to clear DPPH·, O-2·and·OH, and its abilities of scavenging DPPH·and·OH were both less than 50%, and its IC50 of scavenging O-2·was 0.85 mg /m L.IC50 of the components extracted by water for clearing DPPH·, O-2·and ·OH were 0.57, 0.69 and 0.56 mg / m L respectively.The components extracted by ethyl acetate had the ability less than 50% to clear·OH and IC50 of scavenging DPPH·and O-2·were 0.56, 0.62 mg /m L respectively.Therefore, chrysanthemum's extracts in different kinds of solvents all have antioxidant effects, and the antioxidant capacity was related to the extraction solvent.