Optimization of enzymatic hydrolysis conditions for chicken protein by response surface methodology

Chicken bone protein,extracted from chicken bone residue was hydrolyzed by compound protease and the nutritional composition and content of the chicken bone residue were determined in this paper.In single factor experiment,enzyme concentration,hydrolysis temperature and time were selected to detect the better conditions for chicken bone protein hydrolysis with compound protease. Based on the results,by response surface methodology( RSM) and the mathematical model for hydrolysis conditions,the optimal hydrolysis conditions were obtained as: enzyme concentration 1.5%,hydrolysis temperature 55 ℃ and hydrolysis time 2.5 h,then the degree of hydrolysis could reach 25.88%.