Study on extraction process of water soluble polysaccharides from Zizania latifolia and determine component of monosaccharide

For further development and utilization of Zizania latifolia,the extraction conditions of water soluble polysaccharides from Zizania latifolia were studied using monofactorial and orthogonal testing. Monosaccharide components of Zizania latifolia polysaccharides were analyzed by using HPLC.The results showed that the impact of various factors on Zizania latifolia polysaccharides extraction in the order of temperature > time > solid- liquid ratio.The optimum conditions of Zizania latifolia polysaccharides were solid- liquid ratio 1∶40,extraction temperature100 ℃ and extraction time 4 h. Under these conditions,the extraction rate reached 10.69%. Zizania latifolia polysaccharides were mainly composed of galacturonic acid,glucose,galatose and arabinose along with a small amount of mannose,rhamnose and xylose.