Analysis of deoxynivalenol( DON) and masked deoxynivalenol( D3G)accumulation in different treatment wheat grains at room temperature

To elucidate the content of deoxynivalenol( DON) and masked deoxynivalenol( D3G) accumulation and variation in wheat grain. The sterilized wheat grain,with 8 k Gy and 15 k Gy irradiated wheat grain inoculated artificially with Fusarium graminearum strains F-1 and 5035 respectively,were determined by LC- MS / MS after 7,14,21,28 and 35 days of incubation under 25 ℃. The results showed that DON content increased gradually with time in sterilized and 15 k Gy irradiated wheat grain while the content of D3 G were blow limit of detection.The wheat grains can germinate after 8 k Gy irradiation,DON content increased gradually with time in 8 k Gy irradiated wheat grain while the content of D3 G decreased with time. The highest D3 G content was detected in wheat grain incubated after 7 days.The relationship between the ratio of D3 G / DON and incubation time can be well described by exponential model in the conditions of this experiment. R2 of F- 1 and 5035 exponential models regarding the ratio of D3 G / DON and time were 0.97 and 0.93,respectively.As convinced,our results could provide the basis for subsequent studies on the regulatory mechanism of D3 G biosynthesis.