Optimization of enzymatic hydrolysis and determination of scavenging DPPH free radical of seahorse

Seahorse bone powder was hydrolyzed by alkaline protease,the function of the enzymatic hydrolysis of seahorse bone meal by alkaline protease was determined by DPPH method.The response surface method was adopted to obtain the optimum hydrolysis conditions on the basis of results of single- factor experiments.The single- factor experiments showed that the optimum conditions of enzymatic hydrolysis were: time 3 h,temperature50 ℃,and p H8.Central Composite Design was employed to research the further influence of interaction among the3 variables and optimize the enzymatic hydrolysis condition.Design Expert.8.0.5b was used to get regression equation and analyze the optimal condition.The results showed that the optimal condition was: enzyme digestion time was 4 h,digestion temperature was 48.60 ℃,p H value was 7.04,then the predictive scavenging rate was 63.89%.