Study on extraction process of quinoa total flavonoids and purification process of macroporous resin

Purpose:To study the extraction process of quinoa flavonoids and purification process of macroporous resin. Method:To study the optimal extraction and purification process of quinoa through orthogonal test based on single factor test. Results :The optimal extraction process conditions were :ultrasound time for 25 min,temperature 90 ℃,material and liquid ratio 1∶10(g/m L),refluxing time 2 h,ethanol concentration 90%. The total extracting amount of Quinoa flavonoids under these conditions was 3.861 mg/g. Perform salting-out to remove protein. Select Na Cl additive amount as 15%. The optimal purification process of macroporous resin was:p H2,flow velocity 2 BV/h,0.3 mg/m L flavonoids aqueous solution,8 BV water washing,acetone elution. The purified flavonoids adsorption rate under these conditions was 80.91%. The purity could be improved from 7.12% of the original extracts to 28.53%.