Optimization of medium components and fermentation condition for extracellular glucose oxidase production by Penicillium chrysogenum A4

Effects of different kinds of carbon source,organic nitrogen source and inorganic nitrogen source in medium on extracellular glucose oxidase(GOD) production by Penicillium chrysogenum A4 were studied by single-factor tests,respectively. The addition amount of glucose,malt extract and Na NO3 were optimized by orthogonal test. Results showed that the optimal addition amounts were as follow:glucose 10%,malt extract0.5%,Na NO31.7%. Based on the medium with the optimal components,the culture condition was also studied.Results showed that the optimal fermentation condition was as follow:1 m L spore suspension was incubated in50 m L of culture medium and 30 ℃ for 6 days. The glucose oxidase activity was 17.2 U/m L and 17.2 times that of initial enzyme activity.