Effect of high pressure combined thermal treatment on catalase activity of pork

In order to study the effect of different test conditions(200~600 MPa,10~30 min and 30~50 ℃) on the catalase( CAT) activity of pork.Use longissimus muscle as experimental materials and a response surface method(RSM) was applied on the basis of the analysis of the single factor experimental results. The results of RSM showed that pressure and temperature were the most significant factors on the CAT activity. Pressure and temperature as well as pressure and holding time had significant(p<0.01) interactive on CAT activity,while the critical temperatures influencing the activity of CAT linearly decreased with pressure increasing and the critical holding time influencing the activity of CAT linearly increased with pressure increasing. The highest CAT activity processing conditions was 600 MPa,30 ℃,10 min,and its activity was 1.679U/mg prot.