Application of ultraviolet spectrometry in the identification in variety and origin for gordon euryale seeds

Four kinds of extracts( acetic ether, absolute ethyl alcohol, 50% alcohol, 70 ~80 ℃ hot water) were prepared with two kinds of variety(north gordon euryale and South gordon euryale from Tianchang) and origin(north gordon euryale from Tianchang and South gordon euryale from Hubei) gordon euryales as the experimental materials,and then the variances of the ultraviolet absorption spectrum intensity were analysed.Simultaneously,the variances between structural property(hundred-grain weight,diameters of seeds and seed kernel,thickness of seed shell) and active ingredients(total phenol,proanthocyanidins and flavone) of gordon euryale seeds were analyzed. It was discovered in the study that there were all highly significant differences in the maximum peak absorption values of extracts which comed from two kinds of variety and origin gordon euryale seed shell(p≤0.01) in addition to the acetic ether and alcohol extracts of gordon euryale seed shell from different origin,and the variance was extremely remarkable between the diameters of seeds and kernel,thickness of seed shell,proanthocyanidins in kernel and the content of total flavonoids and total phenol in the seed shell(p≤0.01). The search results showed that ultraviolet spectral analysis could have certain discrimination in the variety and origin of gordon euryale,which provided a mind for the identification of gordon euryale variety and origin.