Determination of urotropine in bean curd sticks by stable isotopic dilution- liquid chromatography with tandem mass spectrometry

XU Xing; ZHANG Xiao-ming; ZHANG Yan; SHU Ping; PENG Fei-jin

doi:10.13386/j.issn1002-0306.2015.15.050

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Science and Technology of Food Industry > 2015 > (15): 281-283. > DOI: 10.13386/j.issn1002-0306.2015.15.050

XU Xing, ZHANG Xiao-ming, ZHANG Yan, SHU Ping, PENG Fei-jin. Determination of urotropine in bean curd sticks by stable isotopic dilution- liquid chromatography with tandem mass spectrometry[J]. Science and Technology of Food Industry, 2015, (15): 281-283. DOI: 10.13386/j.issn1002-0306.2015.15.050

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Determination of urotropine in bean curd sticks by stable isotopic dilution- liquid chromatography with tandem mass spectrometry

School of Food Science and Technology,Jiangnan University
Dali Comprehensive Inspection Centre of Quality and Technical Supervision

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Received Date: November 12, 2014

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Abstract

Abstract

A stable isotope dilution mass spectrometry technology for determination of urotropine in bean curd sticks by liquid chromatography with tandem mass spectrometry was developed.Isotopic urotropine was added to bean curd sticks as internal standard,and extracted with acetonitrile.Solid phase extraction( SPE) was applied to purify the sample.The separation was performed on a HILIC column. The analyte was determined by multiple reaction monitoring( MRM) mode with UPLC- MS / MS,and quantified by the internal standard method.The method showed good linear relationship in the range of 1⁴0 μg / L for urotropine,the correlation coefficient square was 0.9996.The limit of quantitation( LOQ) for sample was 2 μg / kg.The average recoveries were 97.8% ¹01.5% at spiked levels of2,10,30 μg / kg.The relative standard deviation( RSD,n = 6) was 1.9% ⁴.8%.This method is sensitive,accurate and reproducible.It is suitable for monitoring illegally added urotropine in bean curd sticks.

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[1]	冼燕萍,陈立伟,罗东辉,等.UPLC-MS/MS测定腐竹和米粉中的乌洛托品[J].江南大学学报:自然科学版,2012,11(1):78-82.
[2]	吴颖,张慧,崔芳,等.超高效液相色谱串联质谱法测定腐竹中乌洛托品的含量[J].食品工业科技,2014,35(17):298-300.
[3]	中华人民共和国卫生部.食品中可能违法添加的非食用物质和易滥用的食品添加剂名单(第四批)[R].2010-3.
[4]	国家质量监督检验检疫总局.SN/T 2226-2008进出口动物源性食品中乌洛托品残留量的检测方法液相色谱-质谱/质谱法[S].北京:中国标准出版社,2008.
[5]	黄国春.气相色谱法测定腐竹中乌洛托品含量的研究[J].广西轻工业,2008(6):26-27.
[6]	温韬,徐强,张少梅,等.高效液相色谱法测定腐竹中的乌洛托品[J].洛阳理工学院学报:自然科学版,2013,23(3):1-4.
[7]	刘春伟,仲雪,马宁.激光拉曼光谱法快速测定腐竹中的微量乌洛托品[J].食品安全质量检测学报,2012,3(4):306-308.
[8]	汪辉,夏立新,彭新凯,等.液相色谱-串联质谱法快速测定食品中的乌洛托品[J].食品与机械,2013,29(3):72-78.
[9]	周秀云,赵勇,俞婧.SPE-UPLC-MS/MS快速测定豆制品中的乌洛托品[J].河北省科学院学报,2013,30(1):64-68.
[10]	张爱芝,张书芬,王全林,等.超高压液相色谱-串联质谱法测定腐竹、米线、年糕中的乌洛托品[J].食品安全质量检测学报,2013,4(2):472-478.
[11]	陆春良,刘娟,刘向农.亲水作用液相色谱电喷雾串联质谱法测定米线中乌洛托品残留[J].扬州大学学报:农业与生命科学版,2013,34(2):82-86.
[12]	Lee SY,Kim BJ,Kim JK.Development of isotope dilutionliquid chromatography tandem mass spectrometry for the accurate determination of fluoroquinolones in animal meat products:Optimization of chromatographic separation for eliminating matrix effects on isotope ratio measurements[J].Journal of Chromatography A,2013,(1277):35-41.
[13]	Hon PYT,Chu PWS,Cheng CH,et al.Development of melamine certified reference material in milk using two different isotope dilution mass spectrometry techniques[J].Journal of Chromatography A,2011,1218(39):6907-6913.
[14]	马雪涛,牛之瑞,冯雷,等.离子交换固相萃取-超高效液相色谱-串联质谱法测定豆制品中乌洛托品残留量[J].食品科学,2014,35(10):166-169.
[15]	徐小民,黄百芬,任一平.豆制品和粉干中乌洛托品的气质联用测定[J].食品安全质量检测学报,2013(3):873-876.

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