Purification and characteristics of SOD in Morchella angusticeps

SOD in Morchella angusticeps was purified by heating and ammonium sulfate graded precipitation.Then,the thermal stability,p H stability,sensitivity and isozyme types of SOD were also studied. Results showed that the best temperature and time of heating method was 60 ℃ and 15 min,respectively. The optimum mass fraction of ammonium sulfate for salt- soluble and salting- out were 40% and 85%,respectively. The purified SOD showed good thermal stability and p H stability. SOD retained more than 50% when save 60 min at 60 ℃ and the accommodation p H was 6⁹.Mn2 +could significantly activate purify SOD,while Fe2 +remarkably inhibit the activity of SOD.The SOD could tolerate hydrogen peroxide,however,it was sensitive to chloroform- ethanol and SDS.Therefore,this SOD belongs to Mn- SOD type.