Optimization of solid fermentation conditions of marine Penicillium expansum for producing salt tolerant pectinase

Objective:To improve the capacity of marine Penicillium expansum for producing salt tolerant pectinase.Methods:Salt tolerance of pectinase from marine Penicillium expansum was investigated. Solid fermentation conditions of pectinase from marine Penicillium expansum were optimized by single factor and orthogonal experiments. Statistical data was analyzed by SPSS 20.0. Results :Pectinase activity was the highest at the concentration of 30g/L Na Cl,and was stable in 0~60g/L Na Cl. The optimal condition for Penicillium expansum was composed of bran 7g,jelly powders 0.42 g,ammonium chloride 0.28 g and artificial seawater 8.4m L,and was cultured at 28℃ for 72 h with 3m L(107cfu/m L) inoculation in each 250 m L triangle flask. The pectinase activity could reach 6643.49U/g. Conclusion :The pectinase had good salt tolerance. Pectinase activity could reach6643.49U/g under optimized conditions,which was 5.36 times more than that at the initial conditions with1239.80U/g.