Study on Geotrichum candidum biomass detection methods in fermentation process

The detection methods of Geotrichum candidum biomass in its fermentation process were discussed in this study. The feasibility of whether the cell components in Geotrichum candidum-ergosterol and glucosamine could be used as indicators for biomass estimation in fermentation was examined and analyzed. The experimental results showed that glucosamine could be a good biomass indicator as it remained constant in cells with the changes of culture time,culture condition and medium. Ergosterol amount changed with the different culture time and medium type;therefore,it could not accurately represent the changes of biomass. The growth curve of Geotrichum candidum measured by glucosamine method was in accordance with the growth curve by dry weight,so it could be able to reflect the changes of Geotrichum candidum biomass accurately. This method was also applied to the process of solid-state fermentation, getting the variation of Geotrichum candidum biomass in its process of solid-state fermentation quickly. So in solid-state fermentation,glucosamine could be used as the indicator to calculate Geotrichum candidum biomass indirectly,which provided the basis for biomass detection in solid-state fermentation.