Cloning and sequence analysis of aromatic aminotransferaseⅠgene from Saccharomyces cerevisiae

Total DNA from Saccharomyces cerevisiae YT0801 was used as the template to clone aromatic aminotransferaseⅠgene YT0801-aro8 by PCR amplification. Bioinformatics softwares were used to analyse the DNA and protein sequence. The result of DNA sequencing showed that the amplified YT0801- aro8 had an open reading frame of 1503 bp,which coded 500 amino acid residues. The molecular mass was 56.2ku and the theoretical isoelectric point was 5.81. Blastn and Blastx alignment results demonstrated that both the nucleotide sequence and the deduced amino acid sequence of YT0801- aro8 exhibited 100 % of similarities to ARO8(Gen Bank Accession number:NM 001181067.1). Besides,the bioinformatics,including physical and chemical properties,the signal peptide,hydrophobicity,hydrophilicity,secondary structure and tertiary structure,were analyzed. The cloning of YT0801- aro8 could provide the molecular base for study of its functions and biosynthesizing 2-phenylethanol.