Effect of extraction methods on the antioxidant activity of crude peptides from Jinhua ham

Crude peptides were extracted by two solutions from Jinhua ham and evaluated for antioxidant activity by radical scavenging,metal ion chelating,reducing power and the total antioxidant capacity(T-AOC) with GSH as control. The results showed that the crude peptide extract from Jinhua ham using PBS as solution(crude peptide P) presented higher peptide content than crude peptide extract using HCl as solution(crude peptide H). The DPPH radical scavenging capacity and the superoxide anion radical scavenging activities of crude peptide P did not differ significantly from that of crude peptide H below 5mg/m L. In addition,the crude peptide P showed higher metal ion chelating activity than crude peptide H and GSH,which presented higher reducing power than crude peptide H at 4mg/m L(p <0.05). Total antioxidant capacity of crude peptide P reached 48% of GSH at 1mg/m L,which was significantly higher than crude peptide H(p<0.05). In conclusion,crude peptide P had higher antioxidant activity than crude peptide H.