Study on detection method of salmonella using aptamer- conjugated microbeads

JIN Na; KONG Xian-li; LIN Min; ZUO Peng

doi:10.13386/j.issn1002-0306.2015.13.058

JIN Na, KONG Xian-li, LIN Min, ZUO Peng. Study on detection method of salmonella using aptamer- conjugated microbeads[J]. Science and Technology of Food Industry, 2015, (13): 321-324. DOI: 10.13386/j.issn1002-0306.2015.13.058

Citation:

Study on detection method of salmonella using aptamer- conjugated microbeads

Graphical Abstract

Abstract

Abstract

Objective: To investigate the feasibility of aptamer- conjugated microbeads for the detection of salmonella.Methods: Amino- terminal aptamer probes were covalently attached onto the surface of microbeads.Fluorescence from microbeads was detected as signal source, and parameters were optimized which may affect detection of the target.Results: Salmonella were detected in six of 20 aquatic product samples by this method, and the detection rate was 30%. Conclusion: The aptamer- conjugated microbeads can be used for high throughput and accurate detection of salmonella.

FullText(HTML)

References (21)

Supplements (0)

Cited By

Science and Technology of Food Industry

Study on detection method of salmonella using aptamer- conjugated microbeads

Abstract

Catalog

Export File

Citation

Format

Content